ProSci

DAZL Antibody

Product Code:
 
PSI-45-462
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-462 (1ug/ml) staining of Rat Testes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-462 (1ug/ml) staining of Rat Testes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-45-462-0.1mg0.1mg£626.00
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Further Information

Additional Names:
DAZL, deleted in azoospermia-like , HGNC:2685, DAZH, DAZL1, DAZLA, MGC26406, SPGYLA, deleted in azoospermia-like autosomal, germline specific RNA binding protein, spermatogenesis gene on the Y-like autosomal
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in Rat Testis lysates (calculated MW of 31.4kDa according to NP_001102884.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Ovary for this particular batch.
Background References:
  • Cauffman G, Van de Velde H, Liebaers I, Van Steirteghem A. DAZL expression in human oocytes, preimplantation embryos and embryonic stem cells. Mol Hum Reprod. 2005 Jun;11(6):405-411. Epub 2005 May 6.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-GNGPQKKSVDR
NCBI Gene ID #:
1618
NCBI Official Name:
deleted in azoospermia-like
NCBI Official Symbol:
DAZL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001342.2
Protein GI Number:
25121978
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q92904