ProSci

CYBR Antibody

Product Code:
 
PSI-45-441
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-441 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stai
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45-441 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stai

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PSI-45-441-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
CYTIP, PSCDBP, CYBR, pleckstrin homology, Sec7 and coiled/coil domains, binding protein, HE, B3-1, CASP, cytohesin binding protein HE, cytohesin-1 interacting protein, pleckstrin homology, Sec7, and coiled-coil domains protein-binding protein, cytohesin 1 interacting protein, cytohesin binder and regulator, pleckstrin homology, Sec7 and coiled-coil domains, binding protein, pleckstrin homology, Sec7 and coiled/coil domains, binding protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38kDa band observed in lysates of cell line Jurkat and approx 40kDa in ysates of cell line MOLT4 (calculated MW of 40kDa according to NP_004279.3). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of A431 and U251 cells. Recommended concentration: 10ug/ml.
Background References:
  • Tang P, Cheng TP, Agnello D, Wu CY, Hissong BD, Watford WT, Ahn HJ, Galon J, Moss J, Vaughan M, O'Shea JJ, Gadina M. Cybr, a cytokine-inducible protein that binds cytohesin-1 and regulates its activity. Proc Natl Acad Sci USA. 2002 Mar 5;99(5):2625-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: SLQRLLQHSSNGN-C
NCBI Gene ID #:
9595
NCBI Official Name:
cytohesin 1 interacting protein
NCBI Official Symbol:
CYTIP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004279.3
Protein GI Number:
47933392
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O60759