Further Information
CIAS1, cold autoinflammatory syndrome 1, AGTAVPRL, AII/AVP, C1orf7, FCAS, FCU, MWS, NALP3, PYPAF1 , AII/AVP receptor-like, Muckle-Wells syndrome, NACHT, LRR and PYD containing protein 3, PYRIN-containing APAF1-like protein 1, angiotensin/vasopressin receptor AII/AVP-like, cryopyrin
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 70kDa band in human lymph node and tonsil lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 118kDa according to NP_004886.3 and 106kDa according to NP_899632.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts. Recommended concentration: 2-4ug/ml.
- Kanneganti TD, Ozoren N, Body-Malapel M, Amer A, Park JH, Franchi L, Whitfield J, Barchet W, Colonna M, Vandenabeele P, Bertin J, Coyle A, Grant EP, Akira S, Nunez G. Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin/Nalp3. Nature. 2006 Mar 9;440(7081):233-6. Epub 2006 Jan 11.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
The immunogen for this antibody is: C-DLYEKAKRDEPK
114548
NLR family, pyrin domain containing 3
NLRP3
Homo sapiens
Liquid
NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1
34878693
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
This antibody is expected to recognise all five reported isoforms (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).
Q96P20