ProSci

Cripto-1 Antibody

Product Code:
 
PSI-45-432
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-432 (2ug/ml) staining of paraffin embedded Mouse Embryo Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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45-432 (2ug/ml) staining of paraffin embedded Mouse Embryo Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

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PSI-45-432-0.1mg0.1mg£626.00
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Further Information

Additional Names:
CFC1, HTX2, CRYPTIC, cripto, FRL-1, cryptic family 1, cripto-1, cryptic, cryptic gene, MGC133213, CR-1, FLJ77897
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 55kDa band in Human Breast, Placenta and Testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.6kDa according to NP_115934.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Mouse Embryonic Liver shows staining of the hepatocyte cell membranes. Recommended concentration: 1-2ug/ml.
Background References:
  • Bianco C, Strizzi L, Ebert A, Chang C, Rehman A, Normanno N, Guedez L, Salloum R, Ginsburg E, Sun Y, Khan N, Hirota M, Wallace-Jones B, Wechselberger C, Vonderhaar BK, Tosato G, Stetler-Stevenson WG, Sanicola M, Salomon DS. Role of human cripto-1 in tumor angiogenesis. J Natl Cancer Inst. 2005 Jan 19;97(2):132-41.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat
Immunogen:
The immunogen for this antibody is: HFTGRYCEHDQRRSEC
NCBI Gene ID #:
55997
NCBI Official Name:
cripto, FRL-1, cryptic family 1
NCBI Official Symbol:
CFC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_115934.1
Protein GI Number:
14211837
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
P0CG37