ProSci

ApoL1 Antibody

Product Code:
 
PSI-46-746
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-746 (0.1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-746 (0.1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-46-746-0.1mg0.1mg£626.00
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Further Information

Additional Names:
APOL1, apolipoprotein L, 1, APO-L, APOL, APOL-I, OTTHUMP00000028705, apolipoprotein L-I, apolipoprotein L1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 45kDa band observed in Human Placenta lysates (calculated MW of 44kDa according to NP_003652.2 and of 45.9kDa according to NP_663318.1 ). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • Perez-Morga D, Vanhollebeke B, Paturiaux-Hanocq F, Nolan DP, Lins L, Homble F, Vanhamme L, Tebabi P, Pays A, Poelvoorde P, Jacquet A, Brasseur R, Pays E. Apolipoprotein L-I promotes trypanosome lysis by forming pores in lysosomal membranes. Science. 2005 Jul 15;309(5733):469-72.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-NNNYKILQADQE
NCBI Gene ID #:
8542
NCBI Official Name:
apolipoprotein L, 1
NCBI Official Symbol:
APOL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003652.2, NP_663318.1
Protein GI Number:
21735614
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Autophagy ,Neuroscience
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_003652.2; NP_663318.1).
Swissprot #:
O14791