ProSci

AMID Antibody

Product Code:
 
PSI-45-248
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-248 (4ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-248 (4ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-45-248-0.1mg0.1mg£626.00
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Further Information

Additional Names:
AIFM2, AMID, PRG3, apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death, 5430437E11Rik, p53-responsive gene 3, apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death, apoptosis-inducing factor, mitochondrion-associated, 2, RP11-367H5.2, OTTHUMP00000019740, apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 48-50kDa band in Human Colon and Duodenum lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.5kDa according to NP_116186. The 48-50kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 4ug/ml.
Background References:
  • Ohiro Y, Garkavtsev I, Kobayashi S, Sreekumar KR, Nantz R, Higashikubo BT, Duffy SL, Higashikubo R, Usheva A, Gius D, Kley N, Horikoshi N. A novel p53-inducible apoptogenic gene, PRG3, encodes a homologue of the apoptosis-inducing factor (AIF). FEBS Lett. 2002 Jul 31;524(1-3):163-71.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: GSQVSVESGALHC
NCBI Gene ID #:
84883
NCBI Official Name:
apoptosis-inducing factor, mitochondrion-associated, 2
NCBI Official Symbol:
AIFM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_116186.1
Protein GI Number:
14318424
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q9BRQ8