ProSci

Neurexin 1 Antibody

Product Code:
 
PSI-46-692
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed with 46-692(0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with 46-692(1mg/ml) in Lane B. Detected
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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed with 46-692(0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with 46-692(1mg/ml) in Lane B. Detected

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PSI-46-692-0.1mg0.1mg£626.00
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Further Information

Additional Names:
neurexin 1, DKFZp313P2036, NRXN1, FLJ35941, Hs.22998, KIAA0578, neurexin I
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing full-length Human NRXN1 (myc and DYKDDDDK tagged), bands of approx. 75 and 60kDa were observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration: 0.5-1ug/ml. Primary incubations were for 1 hour.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
Background References:
  • Feng J, Schroer R, Yan J, Song W, Yang C, Bockholt A, Cook EH Jr, Skinner C, Schwartz CE, Sommer SS. High frequency of neurexin 1beta signal peptide structural variants in patients with autism. Neurosci Lett. 2006 Nov 27;409(1):10-3. Epub 2006 Oct 10.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KEKQPSSAKSSNKN
NCBI Gene ID #:
9378
NCBI Official Name:
neurexin 1
NCBI Official Symbol:
NRXN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004792.1 , NP_620072.1
Protein GI Number:
14149613
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
This antibody is expected to recognise all reported isoforms.
Swissprot #:
P58400