Chemodex

Resazurin sodium salt

Product Code:
 
CDX-R0051
Product Group:
 
Dyes, Stains, and Probes
Supplier:
 
Chemodex
Regulatory Status:
 
RUO
Shipping:
 
Ambient
Storage:
 
+4 °C
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Chemical Structure
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Chemical Structure

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CDX-R0051-G0055 g£74.00
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CDX-R0051-G02020 g£204.00
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This product comes from: Switzerland.
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Further Information

Alternate Names/Synonyms:
Alamar Blue; Vybrant; UptiBlue; 7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt
Appearance:
Black-Violet solid.
CAS:
62758-13-8
EClass:
32160000
Form (Short):
liquid
GHS Symbol:
GHS07
Handling Advice:
Protect from light and moisture.
Hazards:
H315, H319, H335
InChi:
InChI=1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1
InChiKey:
IVGPGQSSDLDOLH-UHFFFAOYSA-M
Long Description:
Chemical. CAS: 62758-13-8. Formula: C12H6NNaO4. MW: 251.17. Synthetic. Fluorescent reagent.
MDL:
MFCD00005036
Molecular Formula:
C12H6NNaO4
Molecular Weight:
251.17
Package Type:
Vial
Precautions:
P261, P305, P351, P338
Product Description:
Resazurin (also known as Alamar Blue), is the N-oxide of the fluorescent dye resorufin. It is useful for detecting reductive activities in cells and has been widely used for measuring cell proliferation and mitochondrial metabolic activity. It is used as a quantifiable detection agent for enzyme activity assays and as an oxidation-reduction indicator in cell proliferation, cell viability and cytotoxicity assays. Resazurin is a non-toxic, water-soluble and redox-sensitive dye that is non-fluorescent until it is reduced to the highly red fluorescent resorufin (lambdaEx/lambdaEm: 563/587 nm). Usually, NADPH or NADH is the reductant that converts resazurin to resorufin in the presence of diaphorase as the enzyme. Thus, resazurin can be used to detect NADH, NADPH, or diaphorase levels. Furthermore, the resazurin/diaphorase/NADPH system can be used to detect any biochemical or enzyme activity that is involved in a biochemical reaction generating NADH or NADPH.
Purity:
~80% (Dye Content) (UV/Vis). Suitable for cell culture application.
Signal word:
Warning
SMILES:
[Na+].[O-]C1=CC=C2C(OC3=CC(=O)C=CC3=[N+]2[O-])=C1
Solubility Chemicals:
Soluble in water (1mg/ml).
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at +4°C.

References

(1) J. O'Brien, et al.; Eur. J. Bioch. 267, 5421 (2000) | (2) D.P. Penney, et al.; Biotech. Histochem. 77, 237 (2002) | (3) S.N. Rampersad; Sensors 12, 12347 (2012) | (4) R.W. Sabnis; Handbook of Fluorescent Dyes and Probes 370 (2015) | (5) M. Koyanagi, et al.; Cytotechnology 68, 1489 (2016)