ProSci

TOPAZ1 Antibody

Product Code:
 
PSI-7737
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
1 / 3
Western blot analysis of TOPAZ1 in human testis tissue lysate with TOPAZ1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
2 / 3
Immunohistochemistry of TOPAZ1 in rat testis tissue with TOPAZ1 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of TOPAZ1 in rat testis tissue with TOPAZ1 antibody at 20 μg/mL.
❮❯

Western blot analysis of TOPAZ1 in human testis tissue lysate with TOPAZ1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of TOPAZ1 in rat testis tissue with TOPAZ1 antibody at 5 μg/mL.
Immunofluorescence of TOPAZ1 in rat testis tissue with TOPAZ1 antibody at 20 μg/mL.

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CodeSizePrice
PSI-7737-0.02mg0.02mg£150.00
Quantity:
PSI-7737-0.1mg0.1mg£449.00
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Further Information

Additional Names:
TOPAZ1 Antibody: C3orf77, C3orf77, Testis- and ovary-specific PAZ domain-containing protein 1
Application Note:
TOPAZ1 antibody can be used for detection of TOPAZ1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
The testis and ovary specific PAZ domain containing 1 (TOPAZ1) protein is a highly conserved protein that is highly expressed in the gonads during germ cell meiosis, suggesting that it may play a role in germ cell development (1). It contains a PAZ domain and a zinc finger domain and is exclusively expressed in adult testis and fetal ovary.
Background References:
  • Baillet A, Le Bouffant R, Volff JN, et al. TOPAZ1, a novel germ cell-specific expressed gene conserved during evolution across vertebrates. PLoS One 2011; 6:e26950.
Buffer:
TOPAZ1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%), Sheep: (88%)
Immunogen:
TOPAZ1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human TOPAZ1.

The immunogen is located within amino acids 1330 - 1380 of TOPAZ1.
NCBI Gene ID #:
375337
NCBI Official Name:
testis and ovary specific PAZ domain containing 1
NCBI Official Symbol:
TOPAZ1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 65, 186 kDa

Observed: 60, 240 kDa
Protein Accession #:
NP_001138502
Protein GI Number:
222537754
Purification:
TOPAZ1 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
TOPAZ1 antibody is human, mouse and rat reactive. At least three isoforms of TOPAZ1 are known to exist.
Swissprot #:
Q8N9V7
User NOte:
Optimal dilutions for each application to be determined by the researcher.