Further Information
WDR5 Antibody: SWD3, BIG-3, BIG3, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein
WDR5 antibody can be used for detection of WDR5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
WDR5 Antibody: WD repeat domain 5 (WDR5) is a member of the WD repeat protein family, which is involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR5, also known as BIG-3, is expressed in the developing growth plate, accelerates chondrocyte and osteoblast differentiation in vitro, and regulates osteoblast differentiation during embryonic bone development. WDR5 interacts with the pluripotency factor Oct4/POU5F1 and is required for the efficient formation of induced pluripotent stem (iPS) cells.
- Smith TF, Gaitatzes C, Saxena K, et al. The WD repeat: a common architecture for diverse functions. Trends Biochem. Sci. 1999; 24:181-5.
- Gori F and Demay MB. BIG-3, a novel WD-40 repeat protein, is expressed in the developing growth plate and accelerates chondrocyte differentiation in vitro. Endocrinology 2004; 145:1050-4.
- Gori F, Friedman LG, and Demay MB. Wdr5, a WD-40 protein, regulates osteoblast differentiation during embryonic bone development. Dev. Biol. 2006; 295:498-506.
- Ang YS, Tsai SY, Lee DF, et al. Wdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network. Cell 2011; 145:183-97.
WDR5 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%), Bovine: (100%)
WDR5 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human WDR5.
The immunogen is located within the first 50 amino acids of WDR5.
11091
WD repeat domain 5
WDR5
Homo sapiens
Liquid
NP_060058
16554627
WDR5 Antibody is affinity chromatography purified via peptide column.
Stem Cell
WDR5 antibody is human specific. WDR5 antibody is predicted to not cross-react with other WDR family members.
P61964
Optimal dilutions for each application to be determined by the researcher.