ProSci

SPRYD2 Antibody

Product Code:
 
PSI-6403
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of SPRYD2 in mouse heart tissue lysate with SPRYD2 antibody at 1 μg/mL.
2 / 3
Immunohistochemistry of SPRYD2 in mouse brain tissue with SPRYD2 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of SPRYD2 in mouse brain tissue with SPRYD2 antibody at 20 μg/mL.
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Western blot analysis of SPRYD2 in mouse heart tissue lysate with SPRYD2 antibody at 1 μg/mL.
Immunohistochemistry of SPRYD2 in mouse brain tissue with SPRYD2 antibody at 2.5 μg/mL.
Immunofluorescence of SPRYD2 in mouse brain tissue with SPRYD2 antibody at 20 μg/mL.

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CodeSizePrice
PSI-6403-0.02mg0.02mg£150.00
Quantity:
PSI-6403-0.1mg0.1mg£449.00
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Further Information

Additional Names:
SPRYD2 Antibody: SPRYD2, TRIM76, C5orf10, DTNBP2, SPRYD2, Cardiomyopathy-associated protein 5, Dystrobrevin-binding protein 2
Application Note:
SPRYD2 antibody can be used for detection of SPRYD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
SPRYD2 Antibody: SPRYD2, also known as Myospryn, was originally identified as the muscle-specific partner of dysbindin and as a Mef-2 target gene. It is a large scaffolding protein localized to the Z-disc/costamere region of striated muscle. SPRYD2 includes a noncanonical tripartite motif (TRIM-like) that lacks the RING domain but consists of a B-box coiled coil (BBC), fibronectin 3 (FN3) repeats, and SPRY domains. SPRYD2 interacts with desmin and calcineurin, and it has been suggested to play a role in the biogenesis of lysosome and negatively regulates slow-fiber-type transformation and skeletal muscle regeneration. SPRYD2 is dysregulated in Duchenne muscular dystrophy.
Background References:
  • Benson MA, Tinsley CL, Blake DJ. Myospryn is a novel binding partner for dysbindin in muscle. J. Biol. Chem. 2004; 279:10450-8.
  • Sarparanta J. Biology of myospryn: what's known J. Muscle Res. Cell Motil. 2008; 29:177-80
  • Durham JT, Brand OM, Arnold M, et al. Myospryn is a direct transcriptional target for MEF2A that encodes a striated muscle, alpha-actinin-interacting, costamere-localized protein. J. Biol. Chem. 2006; 281:6841-9
  • Kielbasa OM, Reynolds JG, Wu CL, et al. Myospryn is a calcineurin-interacting protein that negatively modulates slow-fiber-type transformation and skeletal muscle regeneration. FASEB J. 2011 epub.
Buffer:
SPRYD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SPRYD2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPRYD2.

The immunogen is located within amino acids 3810 - 3860 of SPRYD2.
NCBI Gene ID #:
202333
NCBI Official Name:
cardiomyopathy associated 5
NCBI Official Symbol:
CMYA5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_705838
Protein GI Number:
62241003
Purification:
SPRYD2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
SPRYD2 antibody is predicted to not cross-react with other SPRYD protein family members. At least four isoforms of SPRYD2 are known to exist.
Swissprot #:
Q8N3K9
User NOte:
Optimal dilutions for each application to be determined by the researcher.