ProSci

VASH1 Antibody

Product Code:
 
PSI-6325
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of VASH1 in human brain tissue lysate with VASH1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 3
Immunohistochemistry of VASH1 in mouse brain tissue with VASH1 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of VASH1 in mouse brain tissue with VASH1 antibody at 20 μg/mL.
❮❯

Western blot analysis of VASH1 in human brain tissue lysate with VASH1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of VASH1 in mouse brain tissue with VASH1 antibody at 5 μg/mL.
Immunofluorescence of VASH1 in mouse brain tissue with VASH1 antibody at 20 μg/mL.

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CodeSizePrice
PSI-6325-0.02mg0.02mg£150.00
Quantity:
PSI-6325-0.1mg0.1mg£449.00
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Further Information

Additional Names:
VASH1 Antibody: KIAA1036, KIAA1036, VASH, Vasohibin-1
Application Note:
VASH1 antibody can be used for detection of VASH1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
VASH1 Antibody: VASH1 was originally identified as an endothelium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor that acts in a negative feedback manner. VASH1 mRNA is expressed brain, heart, kidney and placenta in the adult, with robust expression in various organs during embryonic development. VASH1 expression in tumor cells and tumor vasculature is silenced by methylation by increased expression of Zeste homolog 2 (EZH2). The increase of EZH2 expression is induced by VEGF stimulation, leading to poor clinical prognosis. Recombinant adenovirus expressing VASH prevented tumor angiogenesis and inhibited tumor growth, suggesting that it may be a potentially valuable antitumor therapy in the clinic.
Background References:
  • Watanabe K, Hasegawa Y, Yamashita H, et al. Vasohibin as an endothelium-derived negative feedback regulator of angiogenesis. J. Clin. Invest. 2004; 114:989-907
  • Lu C, Han HD, Mangala LS, et al. Regulation of tumor angiogenesis by EZH2. Cancer Cell 2010; 18:185-97.
  • Li D, Zhou K, Wang S, et al. Recombinant adenovirus encoding vasohibin prevents tumor angiogenesis and inhibits tumor growth. Cancer Sci. 2010; 101:448-52.
Buffer:
VASH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
VASH1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human VASH1.

The immunogen is located within amino acids 290 - 340 of VASH1.
NCBI Gene ID #:
22846
NCBI Official Name:
vasohibin 1
NCBI Official Symbol:
VASH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055724
Protein GI Number:
7662454
Purification:
VASH1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
VASH1 antibody is predicted to not cross-react with other vasohibin protein family members. At least two isoforms are known to exist; this antibody will recognize only the long isoform.
Swissprot #:
Q7L8A9
User NOte:
Optimal dilutions for each application to be determined by the researcher.