ProSci

LRRFIP1 Antibody

Product Code:
 
PSI-6219
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of LRRFIP1 in human colon tissue lysate with LRRFIP1 antibody at 1 μg/mL.
2 / 3
Immunohistochemistry of LRRFIP1 in human colon tissue with LRRFIP1 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of LRRFIP1 in human colon tissue with LRRFIP1 antibody at 20 μg/mL.
❮❯

Western blot analysis of LRRFIP1 in human colon tissue lysate with LRRFIP1 antibody at 1 μg/mL.
Immunohistochemistry of LRRFIP1 in human colon tissue with LRRFIP1 antibody at 5 μg/mL.
Immunofluorescence of LRRFIP1 in human colon tissue with LRRFIP1 antibody at 20 μg/mL.

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CodeSizePrice
PSI-6219-0.02mg0.02mg£150.00
Quantity:
PSI-6219-0.1mg0.1mg£449.00
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Further Information

Additional Names:
LRRFIP1 Antibody: GCF2, TRIP, FLAP1, GCF-2, FLAP-1, HUFI-1, FLIIAP1, GCF2, Leucine-rich repeat flightless-interacting protein 1, GC-binding factor 2, LRR FLII-interacting protein 1
Application Note:
LRRFIP1 antibody can be used for detection of LRRFIP1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
LRRFIP1 Antibody: LRRFIP1, also known as GC-binding factor 2 (GCF2), is a 738 amino acid transcriptional repressor that mainly plays a cytoskeletal role. It is primarily localized in the cytoplasm and preferentially binds to GC-rich dsDNA, but will also bind directly to dsRNA as well. The RNA binding domain encompasses a lysine-rich motif. LRRFIP1 interacts with the mammalian Flightless I (Fli-I) and is a key component in the cytoskeletal regulation of platelet function. LRRFIP1 and the related protein LRRFIP2 may modulate canonical WNT signaling and mediate the IRF3-induced production of type I interferon via a beta-catenin-dependent pathway.
Background References:
  • Rikiyama T, Curtis J, Oikawa M, et al. GCF2: expression and molecular analysis of repression. Biochim. Biophys. Acta 2003; 1629:15-25.
  • Suriano AR, Sanford AN, Kim N, et al. GCF2/LRRFIP1 represses tumor necrosis factor α expression. Mol. Cell. Biol. 2005; 25:9073-81.
  • Wilson SA, Brown EC, Kingsman AJ, et al. TRIP: a novel double-stranded RNA binding protein which interacts with the leucine-rich repeat of Flightless I. Nucleic Acids Res. 1998; 26:3460-7.
  • Goodall AH, Burns P, Salles I, et al. Transcription profiling in human platelets reveals LRRFIP1 as a novel protein regulating platelet function. Blood 2010; 116:4646-56.
Buffer:
LRRFIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LRRFIP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human LRRFIP1.

The immunogen is located within the last 50 amino acids of LRRFIP1.
NCBI Gene ID #:
9208
NCBI Official Name:
leucine rich repeat (in FLII) interacting protein 1
NCBI Official Symbol:
LRRFIP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001131024
Protein GI Number:
212276096
Purification:
LRRFIP1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
LRRFIP1 antibody is predicted to not cross-react with other LRRFIP family members. Three isoforms of LRRFIP1 are known to exist; this antibody will only recognize the two longer isoforms.
Swissprot #:
Q32MZ4
User NOte:
Optimal dilutions for each application to be determined by the researcher.