Further Information
TFF3 Antibody: ITF, P1B, TFI, ITF, Trefoil factor 3, Intestinal trefoil factor, hITF
TFF3 antibody can be used for detection of TFF3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry at starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
TFF3 Antibody: Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.
- Podolsky DK, Lynch-Devaney K, Stow JL, et al. Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion. J. Biol. Chem. 1993; 268:6694-702.
- Gott P, Beck S, Machado JC, et al. Human trefoil peptides: genomic structure in 21q22.3 and coordinated expression. Eur. J. Hum. Genet. 1996; 4:308-15.
- Hernandez C, Santamatilde E, McCreath KJ, et al. Induction of trefoil factor TFF1, TFF2 and TFF3 by hypoxia is mediated by hypoxia inducible factor-1: implications for gastric mucosal healing. Br. J. Pharmacol. 2009; 156:262-72.
- Thim L. Trefoil peptides: from structure to function. Cell Mol. Life Sci. 1997; 53:888-903.
TFF3 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine: (81%), Pig: (81%)
TFF3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TFF3.
The immunogen is located within the last 50 amino acids of TFF3.
7033
trefoil factor 3 (intestinal)
TFF3
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 10 kDa
Observed: 11 kDa
NP_003217
119629977
TFF3 Antibody is affinity chromatography purified via peptide column.
Homeostasis
TFF3 antibody is predicted to not cross-react with other TFF family members.
Q07654
Optimal dilutions for each application to be determined by the researcher.