Further Information
KLOTHO Antibody: Klotho
WB: 1-4 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
KLOTHO Antibody: KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.
- Kuro-o et al. Nature1997; 390:45-51.
- Kurosu et al. Science2005; 309:1829-33.
- Liu et al. Science 2007; 317:803-6.
- Wolf et al. Oncogene2008; 27:7094-105.
KLOTHO Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Anti-KLOTHO antibody (6107) was raised against a peptide corresponding to 17 amino acids near the center of human KLOTHO.
The immunogen is located within amino acids 410-460 of KLOTHO.
Human KLOTHO has 2 isoforms, including isoform 1 (1012aa, 116kD) and isoform 2 (549aa, 62kD). Mouse KLOTHO has 2 isoforms, including isoform 1 (1014aa, 116kD) and isoform 2 (550aa, 62kD). Rat KLOTHO has one isoform (1014aa, 117kD). 6107 can detect human, mouse and rat.
9365
klotho
KL
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 116kD
Observed: 116kD
NP_004786
24497614
KLOTHO Antibody is affinity chromatography purified via peptide column.
Signal Transduction
Three isoforms of KLOTHO are known to exist.
Q9UEF7
Optimal dilutions for each application to be determined by the researcher.
Independent Antibody Validation in Cell lines (Figure 1) shows similar KLOTHO expression profile in human and mouse cell lines detected by two independent anti-KLOTHO antibodies that recognize different epitopes, 6107 against central domain and competitor antibody against human recombinant protein. KLOTHO proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.
Recombinant Protein Test (Figure 2): Anti-KLOTHO antibodies (6107) detected human KLOTHO recombinant protein at different concentrations.