ProSci

PPAPDC2 Antibody

Product Code:
 
PSI-5517
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
1 / 2
Western blot analysis of PPAPDC2 in Raji cell lysate with PPAPDC2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 2
Immunofluorescence of PPAPDC2 in Raji cells with PPAPDC2 antibody at 20 μg/mL.
❮❯

Western blot analysis of PPAPDC2 in Raji cell lysate with PPAPDC2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of PPAPDC2 in Raji cells with PPAPDC2 antibody at 20 μg/mL.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-5517-0.02mg0.02mg£150.00
Quantity:
PSI-5517-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Related Products
  • Show All

Further Information

Additional Names:
PPAPDC2 Antibody: PDP1, PSDP, bA6J24.6, Presqualene diphosphate phosphatase, Phosphatidic acid phosphatase type 2 domain-containing protein 2, PPAP2 domain-containing protein 2
Application Note:
PPAPDC2 antibody can be used for detection of PPAPDC2 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PPAPDC2 Antibody: PPAPDC2 is a phosphatase that dephosphorylates Presqualene diphosphate (PSDP) into presqualene monophosphate (PSMP), suggesting that it may have important role in innate immunity. PSDP is a bioactive lipid that rapidly remodels to PSMP upon cell activation. PPAPDC2 displays diphosphate phosphatase activity with a substrate preference for PSDP > FDP > phosphatidic acid. PPAPDC2 activity is independent of Mg2+ and has been identified in activated human neutrophil (PMN) extracts. It is widely expressed in human tissues. Recent studies shows PPAPDC2 is a functional isoprenoid diphosphate phosphatase.
Background References:
  • Fukunaga K, Arita M, Takahashi M, et al. Identification and functional characterization of a presqualene diphosphate phosphatase. J. Biol. Chem.2006; 281:9490-7.
  • Miriyala S, Subramanian T, Panchatcharam M, et al. Functional characterization of the atypical integral membrane lipid phosphatase PDP1/PPAPDC2 identifies a pathway for interconversion of isoprenols and isoprenoid phosphates in mammalian cells. J. Biol. Chem.2010 (epub).
Buffer:
PPAPDC2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%)
Immunogen:
PPAPDC2 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus human PPAPDC2.

The immunogen is located within amino acids 170 - 220 of PPAPDC2.
NCBI Gene ID #:
403313
NCBI Official Name:
phosphatidic acid phosphatase type 2 domain containing 2
NCBI Official Symbol:
PPAPDC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_982278
Protein GI Number:
66773040
Purification:
PPAPDC2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
Q8IY26
User NOte:
Optimal dilutions for each application to be determined by the researcher.