ProSci

PPAPDC1A Antibody

Product Code:
 
PSI-5513
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
1 / 2
Western blot analysis of PPAPDC1A in human brain tissue lysate with PPAPDC1A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 2
Immunofluorescence of PPAPDC1A in human brain tissue with PPAPDC1A antibody at 20 μg/mL.
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Western blot analysis of PPAPDC1A in human brain tissue lysate with PPAPDC1A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of PPAPDC1A in human brain tissue with PPAPDC1A antibody at 20 μg/mL.

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CodeSizePrice
PSI-5513-0.02mg0.02mg£150.00
Quantity:
PSI-5513-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
PPAPDC1A Antibody: DPPL2, PPAPDC1, DPPL2, Phosphatidate phosphatase PPAPDC1A, Phosphatidic acid phosphatase type 2 domain-containing protein 1A
Application Note:
PPAPDC1A antibody can be used for detection of PPAPDC1A by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PPAPDC1A Antibody: Phosphatidate phosphatase (PAP) plays important role in lipid-signaling metabolism in eukaryotic cells. Two distinct types of PAP (PAP1 and PAP2) activity have been distinguished by their subcellular localization and differential sensitivity to N-ethylmaleimide(NEM) and Mg2+. A yeast diacylglycerol pyrophosphate (DGPP) phosphatase (DPP1) and mammalian DGPP phosphatase (PAP2) have been identified as Mg2+-independent and NEM-insensitive membrane-associated. PPAPDC1A (also known as DPPL2) and PPAPDC1B (DPPL1) form a novel type of Mg2+-independent and NEM-sensitive mammalian phosphatidate phosphatase showing broad substrate specificity. PPAPDC1A is preferentially expressed in endothelial cells. Studies of PPAPDC1A and PAP activity suggest that they may play a role in angiogenesis.
Background References:
  • Takeuchi M, Harigai M, Momohara S, et al. Cloning and characterization of DPPL1 and DPPL2, representatives of a novel type of mammalian phosphatidate phosphatase. Gene2007; 399:174-80.
  • Bernard-Pierrot I, Gruel N, Stransky N, et al. Characterization of the recurrent 8p11-12 amplicon identifies PPAPDC1B, a phosphatase protein, as a new therapeutic target in breast cancer. Cancer Res.2008; 68:7165-75.
Buffer:
PPAPDC1A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PPAPDC1A antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human PPAPDC1A.

The immunogen is located within the last 50 amino acids of PPAPDC1A.
NCBI Gene ID #:
196051
NCBI Official Name:
phosphatidic acid phosphatase type 2 domain containing 1A
NCBI Official Symbol:
PPAPDC1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001025230
Protein GI Number:
73611920
Purification:
PPAPDC1A Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q5VZY2
User NOte:
Optimal dilutions for each application to be determined by the researcher.