ProSci

THEM4 Antibody

Product Code:
 
PSI-5227
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of THEM4 in human liver tissue lysate with THEM4 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunohistochemistry of THEM4 in human liver tissue with THEM4 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of THEM4 in human liver tissue with THEM4 antibody at 20 μg/mL.
❮❯

Western blot analysis of THEM4 in human liver tissue lysate with THEM4 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of THEM4 in human liver tissue with THEM4 antibody at 2.5 μg/mL.
Immunofluorescence of THEM4 in human liver tissue with THEM4 antibody at 20 μg/mL.

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CodeSizePrice
PSI-5227-0.02mg0.02mg£150.00
Quantity:
PSI-5227-0.1mg0.1mg£449.00
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Further Information

Additional Names:
THEM4 Antibody: CTMP, CTMP, Acyl-coenzyme A thioesterase THEM4, Carboxyl-terminal modulator protein, Acyl-CoA thioesterase THEM4
Application Note:
THEM4 antibody can be used for detection of THEM4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
THEM4 Antibody: THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.
Background References:
  • Maira SM, Galetic I, Brazil DP, et al. Carboxyl-terminal modulator protein (CTMP), a negative regulator of PKB/Akt and v-Akt at the plasma membrane. Science2001; 294:374-80.
  • Knobbe CB, Reifberger J, Blaschke B, et al. Hypermethylation and transcriptional downregulation of the carboxyl-terminal modulator protein gene in glioblastomas. J. Natl. Cancer Inst.2004; 96:483-6.
  • Zhao H, Martin BM, Bisoffi M, et al. The Akt C-terminal modulator protein is an acyl-CoA thioesterase of the hotdog-fold family. Biochemistry2009; 48:5507-9.
  • Parcellier A, Tintignac LA, Zhuravleva E, et al. Carboxy-terminal modulator protein (CTMP) is a mitochondrial protein that sensitizes cells to apoptosis. Cell Signal.2009; 21:639-50.
Buffer:
THEM4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
THEM4 antibody was raised against an 18 amino acid synthetic peptide near the center of human THEM4.

The immunogen is located within amino acids 60 - 110 of THEM4.
NCBI Gene ID #:
117145
NCBI Official Name:
thioesterase superfamily member 4
NCBI Official Symbol:
THEM4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_444283
Protein GI Number:
76159293
Purification:
THEM4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.
Swissprot #:
Q5T1C6
User NOte:
Optimal dilutions for each application to be determined by the researcher.