ProSci

Alpha-tubulin Antibody

Product Code:
 
PSI-5103
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Chicken
Antibody Isotype:
 
IgY
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
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Western blot analysis of Tubulin in rat brain tissue lysate with Tubulin antibody at (A) 0.5 and (B) 1 μg/mL.
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Western blot analysis of Tubulin in rat brain tissue lysate with Tubulin antibody at (A) 0.5 and (B) 1 μg/mL.

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CodeSizePrice
PSI-5103-0.02mg0.02mg£150.00
Quantity:
PSI-5103-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
Alpha-tubulin Antibody: TUBA2, bA408E5.3, TUBA2, TUBA3D, Alpha-tubulin 2
Application Note:
Tubulin antibody can be used for detection of Tubulin by Western blot at 0.5 - 1 μg/mL.

Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.
Background:
Alpha-tubulin Antibody: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots.
Background References:
  • McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci.2001; 114:2723-33.
  • Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol.1988; 2:133-53.
  • Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem.2009; 109:683-693.
Buffer:
Alpha-tubulin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%), Chicken: (100%)
Immunogen:
Tubulin antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human Tubulin.

The immunogen is located within amino acids 140 - 190 of Alpha-tubulin.
NCBI Gene ID #:
7278
NCBI Official Name:
tubulin, alpha 3c
NCBI Official Symbol:
TUBA3C
NCBI Organism:
homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005992
Protein GI Number:
17921993
Purification:
Alpha-tubulin Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q13748
User NOte:
Optimal dilutions for each application to be determined by the researcher.