Ambra1 Antibody

ProSci
Product Code: PSI-4555
Product Group: Primary Antibodies
Supplier: ProSci

CodeSizePrice
PSI-4555-0.02mg0.02mg£150.00
Quantity:
PSI-4555-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of Ambra1 in 3T3 cell lysate with Ambra1 antibody at 1 μg/mL.
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Immunohistochemistry of Ambra1 in human brain with Ambra1 antibody at 5 μg/mL.
3 / 4
Immunofluorescence of Ambra1 in Human Brain cells with Ambra1 antibody at 20 μg/mL.
4 / 4
Immunofluorescence of Ambra1 in K562 cells with Ambra1 Antibody at 20 μg/ml.

Western blot analysis of Ambra1 in 3T3 cell lysate with Ambra1 antibody at 1 μg/mL.
Immunohistochemistry of Ambra1 in human brain with Ambra1 antibody at 5 μg/mL.
Immunofluorescence of Ambra1 in Human Brain cells with Ambra1 antibody at 20 μg/mL.
Immunofluorescence of Ambra1 in K562 cells with Ambra1 Antibody at 20 μg/ml.

Further Information

Additional Names:
Ambra1 Antibody: DCAF3, WDR94, KIAA1736, Activating molecule in BECN1-regulated autophagy protein 1
Application Note:
Ambra1 antibody can be used for the detection of Ambra1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Ambra1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.
Background References:
  • Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906.
  • Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.
  • Liang XH, Jackson S, Seaman M, et al. Induction of autophagy and inhibition of tumorigenesis by beclin 1. Nature1999; 402:672-6.
  • Fimia GM, Stoykova A, Romagnoli A, et al. Ambra1 regulates autophagy and development of the nervous system. Nature2007; 447:1121-5.
Buffer:
Ambra1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ambra1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human Ambra1.

The immunogen is located within the last 50 amino acids of Ambra1.
NCBI Gene ID #:
55626
NCBI Official Name:
autophagy/beclin-1 regulator 1
NCBI Official Symbol:
AMBRA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9C0C7
Protein GI Number:
166215833
Purification:
Ambra1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
Swissprot #:
Q9C0C7
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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