ProSci

TEM4 Antibody

Product Code:
 
PSI-4367
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 2
Western blot analysis of TEM4 in Jurkat cell lysate with TEM4 antibody at (A) 0.5 and (B) 1 μg/mL.
2 / 2
Immunohistochemistry of TEM4 in human spleen tissue with TEM4 antibody at 2.5 μg/mL.
❮❯

Western blot analysis of TEM4 in Jurkat cell lysate with TEM4 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of TEM4 in human spleen tissue with TEM4 antibody at 2.5 μg/mL.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-4367-0.02mg0.02mg£150.00
Quantity:
PSI-4367-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Related Products
  • Show All

Further Information

Additional Names:
TEM4 Antibody: TEM4, P164RHOGEF, p164-RhoGEF, KIAA0337, TEM4, Rho guanine nucleotide exchange factor 17, 164 kDa Rho-specific guanine-nucleotide exchange factor
Application Note:
TEM4 antibody can be used for detection of TEM4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
TEM4 Antibody: Rho GTPases, which are activated by specific guanine-nucleotide exchange factors (GEFs), play pivotal roles in several cellular functions. TEM4, encoding a protein containing 1510 amino acids, contains a RhoGEF-specific Dbl homology (DH) domain but lacks their typical pleckstrin homology domain. TEM4 is a Rho-specific GEF with novel structural and regulatory properties and predominant expression in the heart. It couples tyrosine kinase signals with the activation of the rho/rac GTPases, thus leading to cell differentiation and/or proliferation. Elevated levels of TEM4, TEM5, TEM6, TEM7 and TEM7R were also raised in breast cancer tissues. TEM4 could also prove to be useful targets therapeutically.
Background References:
  • Ruemenapp U, Freichel-Blomquist A, Wittinghofer B, et al. A mammalian Rho-specific guanine-nucleotide exchange factor (p164-RhoGEF) without a pleckstrin homology domain. Biochem. J.2002; 366:721-8.
  • Carson-Walter EB, Watkins DN, Nanda A, et al. Cell surface tumor endothelial markers are conserved in mice and humans. Cancer Res.2001; 61:6649-55.
  • Davies G, Cunnick GH, Mansel RE, et al. Levels of expression of endothelial markers specific to tumour-associated endothelial cells and their correlation with prognosis in patients with breast cancer. Clinical & Experimental Metastasis2004; 21:31-7.
  • Nanda A and St Croix B. Tumor endothelial markers: new targets for cancer therapy. Curr. Opin. Oncol.2004; 16:44-9.
Buffer:
TEM4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (94%)
Immunogen:
TEM4 antibody was raised against a 18 amino acid synthetic peptide near the center of human TEM4.

The immunogen is located within amino acids 910 - 960 of TEM4.
NCBI Gene ID #:
9828
NCBI Official Name:
Rho guanine nucleotide exchange factor (GEF) 17
NCBI Official Symbol:
ARHGEF17
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 220 kDa

Observed: 200 kDa
Protein Accession #:
Q96PE2
Protein GI Number:
74724250
Purification:
TEM4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q96PE2
User NOte:
Optimal dilutions for each application to be determined by the researcher.