ProSci

LKB1 Antibody

Product Code:
 
PSI-3987
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
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Western blot analysis of LKB1 in PC-3 cell lysate with LKB1 antibody at 1 μg/mL in the (A) absence or (B) presence of blocking peptide.
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Western blot analysis of LKB1 in PC-3 cell lysate with LKB1 antibody at 1 μg/mL in the (A) absence or (B) presence of blocking peptide.

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CodeSizePrice
PSI-3987-0.02mg0.02mg£150.00
Quantity:
PSI-3987-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
LKB1 Antibody: PJS, LKB1, hLKB1, PJS, Liver kinase B1
Application Note:
LKB1 antibody can be used for detection of LKB1 by Western blot at 1 μg/mL. LKB1 often migrates at a higher than expected molecular weight in SDS-PAGE.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
LKB1 Antibody: The LKB1 serine/threonine protein kinase was initially identified as a tumor suppressor gene mutated in human Peutz-Jeghers syndrome (PJS), a condition resulting in the growth of numerous intestinal polyps classed as hamartomas. LKB1 exists as a heterotrimeric complex with two other proteins, Ste20-related adaptor protein (STRAD) and MO25. Together, this complex can phsophorylate and activate the AMP-activate protein kinase (AMPK). Following AMPK activation by LKB1, AMPK then phosphorylates TSC1 and TSC2, key components of the metabolism-regulating TOR signaling pathway, which antagonizes the activation for the TOR pathway. LKB1 has also been shown to play a fundamental role in controlling the spatial orientation of structures required to maintain an ordered, polarized epithelium.
Background References:
  • Hemminki A, Markie D, Tomlinson I, et al. A serine/threonine kinase gene defective in Peutz-Jeghers syndrome. Nature 1998; 391:184-7.
  • Boudeau J, Baas AF, Deak M, et al. MO25α/β interact with STRADα/β enhancing their ability to bind, activate and localize LKB1 in the cytoplasm. EMBO J. 2003; 22:5102-14.
  • Hawley SA, Boudeau J, Reid JL, et al. Complexes between the LKB1 tumor suppressor, STRADα/β and MO25α/β are upstream kinases in the AMP-activated protein kinase cascade. J. Biol. 2003; 2:28
Buffer:
LKB1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Chicken: (87%)
Immunogen:
LKB1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human LKB1.

The immunogen is located within amino acids 310 - 360 of LKB1.
NCBI Gene ID #:
6794
NCBI Official Name:
serine/threonine kinase 11
NCBI Official Symbol:
STK11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAH19334
Protein GI Number:
17939640
Purification:
LKB1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Cancer
Swissprot #:
Q15831
User NOte:
Optimal dilutions for each application to be determined by the researcher.