Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
1 / 2
2 / 2
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Further Information
SkiP Antibody: Bx42, SKIP, Prp45, SKIIP, PRPF45, NCOA-62, SNW domain-containing protein 1, Nuclear protein SkiP
SkiP antibody can be used for detection of SkiP by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SkiP Antibody: TGF-beta and the bone morphogenic proteins (BMPs) are key signaling proteins that regulate numerous cellular processes such as embryonic development and tumorigenesis. Both signal through the Smad protein family and are negatively regulated by Ski and SnoN, two related proto-oncoproteins. Ski functions by binding to the Smad proteins activated by TGF-beta and the (BMPs) and preventing their phosphorylation, inhibiting their ability to bind DNA and activate the transcription of downstream genes. SkiP was originally identified as a Ski-interacting protein and was later found to augment the signals induced by TGF-beta but inhibit transcription induced by BMP-2 in C2C12 cells, suggesting that SkiP is a key player in the signaling cascades inititated by TGF-beta and the BMP protein family.
- Derynck R, Akhurst RJ, and Balmain A. TGF-β signaling in tumor suppression and cancer progression. Nat. Genet. 2001; 29:117-129.
- Li Y, Turck CM, Teumer JK, et al. Unique sequence, SkiP, in Sloan-Kettering avian retrovirus with properties of a new cell-derived oncogene. J. Virol. 1986; 57:1065-72.
- Luo K. SkiP and SkiP: negative regulators of TGF-β signaling. Curr. Op. Gen. Dev. 2004; 14:65-70.
- Massague J and Wotton D. Transcriptional control by the TGF-b/Smad signaling system. EMBO J. 2000; 19:1745-54.
SkiP Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/ml
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
SkiP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human SkiP.
The immunogen is located within the last 50 amino acids of SkiP.
22938
SNW domain containing 1
SNW1
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 58, 62 kDa
Observed: 65 kDa?
NP_036377
6912676
SkiP Antibody is affinity chromatography purified via peptide column.
Signal Transduction
Q13573
Optimal dilutions for each application to be determined by the researcher.