ProSci

ASK1 Antibody

Product Code:
 
PSI-3677
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of ASK1 in SW1353 cell lysate with ASK1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
2 / 3
Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 2 μg/mL.
3 / 3
Immunofluorescence of ASK1 in 3T3 cells with ASK1 antibody at 20 μg/mL.
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Western blot analysis of ASK1 in SW1353 cell lysate with ASK1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 2 μg/mL.
Immunofluorescence of ASK1 in 3T3 cells with ASK1 antibody at 20 μg/mL.

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CodeSizePrice
PSI-3677-0.02mg0.02mg£150.00
Quantity:
PSI-3677-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
ASK1 Antibody: ASK1, MEKK5, MAPKKK5, ASK1, Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, ASK-1
Application Note:
ASK1 antibody can be used for the detection of ASK1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
ASK1 Antibody: Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-α-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.
Background References:
  • Ichijo H, Nishida E, Irie K, et al. Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science1997; 275:90-4.
  • Wang XS, Diener K, Jannuzzi D, et al. Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase. J. Biol. Chem.1996; 271:31607-11.
Buffer:
ASK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ASK1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ASK1.

The immunogen is located within amino acids 1260 - 1310 of ASK1.
NCBI Gene ID #:
4217
NCBI Official Name:
mitogen-activated protein kinase kinase kinase 5
NCBI Official Symbol:
MAP3K5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q99683
Protein GI Number:
6685617
Purification:
ASK1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
Q99683
User NOte:
Optimal dilutions for each application to be determined by the researcher.