Caspase-12 Antibody (Large)
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
1 / 8
2 / 8
3 / 8
4 / 8
5 / 8
6 / 8
7 / 8
8 / 8
❮❯No additional charges, what you see is what you pay! *
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available!
Contact us to find what you can save.
This product comes from:
United States.
Typical lead time:
14-21 working days.
Contact us for more accurate information.
- Further Information
- Documents
- Related Products
- Show All
Further Information
Caspase-12 (Large) Antibody: Caspase-12, CASP-12
Caspase-12 (large) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 0.25 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human, mouse, and rat samples and Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
Caspase-12 Antibody: (Large)Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
- Nakagawa T, Zhu H, Morishima N, et al. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 2000; 403:98-103.
- Mehmet H. Caspases find a new place to hide. Nature 2000; 403:29-30
- Van de Craen M, Vandenabeele P, Declercq W, et al. Characterization of seven murine caspase family members. FEBS Lett. 1997; 403:61-9
Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Large)
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Caspase-12 antibody was raised against a synthetic peptide corresponding to 16 amino acids within the large cleavage product of murine caspase-12.
The immunogen is located within amino acids 170 - 220 of Caspase-12.
12364
caspase 12
Casp12
Mus musculus
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 46 kDa
Observed: 46 kDa
CAA73532
2094806
Caspase-12 (Large) Antibody is affinity chromatography purified via peptide column.
Apoptosis,Cancer
Caspase-12 (large) antibody is predicted to have no cross reactivity to other members in the caspase family.
O08736
Optimal dilutions for each application to be determined by the researcher.