Further Information
PHAP I Antibody: LANP, MAPM, PP32, HPPCn, PHAP1, PHAPI, I1PP2A, C15orf1, LANP, Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32
WB: 1 μg/mL; IHC: 2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
PHAP I Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
- Jiang et al. Science. 2003;299(5604):223-6.
- Nicholson and Thornberry. Science. 2003 10;299(5604):214-5.
PHAP I Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine: (85%)
Anti-PHAP I antibody (3151) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human PHAP I .
The immunogen is located within the last 50 amino acids of PHAP I.
8125
acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
ANP32A
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 29kD
Observed: 29 kD
P39687
730318
PHAP I Antibody is DEAE purified.
Apoptosis
This polyclonal antibody has no cross-reaction to PHAP I2a and PHAP III.
P39687
Optimal dilutions for each application to be determined by the researcher.