ProSci

Noxa Antibody

Product Code:
 
PSI-2437
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 4
Western blot analysis of Noxa in human stomach tissue lysate with Noxa antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
2 / 4
Immunohistochemistry of NOXA in human stomach tissue with NOXA antibody at 2.5 μg/ml.
3 / 4
Immunofluorescence of Noxa in Human Stomach cells with Noxa antibody at 10 μg/mL.
4 / 4
Immunohistochemistry of Noxa in human stomach tissue with Noxa antibody at 1 μg/mL.
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Western blot analysis of Noxa in human stomach tissue lysate with Noxa antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunohistochemistry of NOXA in human stomach tissue with NOXA antibody at 2.5 μg/ml.
Immunofluorescence of Noxa in Human Stomach cells with Noxa antibody at 10 μg/mL.
Immunohistochemistry of Noxa in human stomach tissue with Noxa antibody at 1 μg/mL.

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CodeSizePrice
PSI-2437-0.02mg0.02mg£150.00
Quantity:
PSI-2437-0.1mg0.1mg£449.00
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Further Information

Additional Names:
Noxa Antibody: Noxa, Phorbol-12-myristate-13-acetate-induced protein 1
Application Note:
Noxa antibody can be used for detection of Noxa by Western blot at at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Noxa Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified, which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9. Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53. Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development.
Background References:
  • Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science 2000; 288:1053-8.
  • Nakano K and Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol. Cell 2001; 7:683-94.
  • Yu J, Zhang L, Hwang PM, et al. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol. Cell 2001; 7:673-82.
  • Eferl R, Ricci R, Kenner L, et al. Liver tumor development. c-Jun antagonizes the proapoptotic activity of p53. Cell 2003; 112:181-92.
Buffer:
Noxa Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Noxa antibody was raised against a synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa.

The immunogen is located within the first 50 amino acids of Noxa.
NCBI Gene ID #:
58801
NCBI Official Name:
phorbol-12-myristate-13-acetate-induced protein 1
NCBI Official Symbol:
Pmaip1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_067426
Protein GI Number:
10946832
Purification:
Noxa Antibody is immunoaffinity chromatography purified IgG.
Research Area:
Apoptosis,Cancer
Swissprot #:
Q9JM54
User NOte:
Optimal dilutions for each application to be determined by the researcher.