ProSci

EndoG Monoclonal Antibody

Product Code:
 
PSI-PM-4583
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
7F2G10
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
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Western blot analysis of EndoG expression in HepG2 cell lysate with EndoG antibody at (A) 5 and (B) 10 μg/mL.
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Western blot analysis of EndoG expression in HepG2 cell lysate with EndoG antibody at (A) 5 and (B) 10 μg/mL.

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CodeSizePrice
PSI-PM-4583-0.02mg0.02mg£150.00
Quantity:
PSI-PM-4583-0.1mg0.1mg£515.00
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Further Information

Additional Names:
EndoG Antibody [7F2G10] : Endo G
Application Note:
EndoG monoclonal monoclonal antibody can be used for detection of EndoG by Western blot at 5 - 10 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
EndoG Monoclonal Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.
Background References:
  • Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature 2001; 412:95-9.
  • Parrish J, Li L, Klotz K, et al. Mitochondrial endonuclease G is important for apoptosis in C. elegans. Nature 2001; 412:90-4
  • Hengartner MO. Apoptosis. DNA destroyers. Nature 2001; 412:27, 29.
  • Widlak P, Li LY, Wang X, et al. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J. Biol. Chem. 2001; 276:48404-9.
Buffer:
EndoG Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein corresponding to amino acids 51 - 140 of human EndoG.
NCBI Gene ID #:
2021
NCBI Official Name:
endonuclease G
NCBI Official Symbol:
ENDOG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q14249
Protein GI Number:
24638471
Purification:
EndoG Monoclonal Antibody is Protein A purified.
Research Area:
Apoptosis
Swissprot #:
Q14249
User NOte:
Optimal dilutions for each application to be determined by the researcher.