ProSci

PUMA Monoclonal Antibody

Product Code:
 
PSI-PM-4529
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
10D4G7
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
1 / 1
Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at (A) 2.5 and (B) 5 μg/mL.
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Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at (A) 2.5 and (B) 5 μg/mL.

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CodeSizePrice
PSI-PM-4529-0.02mg0.02mg£150.00
Quantity:
PSI-PM-4529-0.1mg0.1mg£515.00
Quantity:
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Further Information

Additional Names:
PUMA Antibody [10D4G7] : JFY1, PUMA, JFY-1
Application Note:
PUMA antibody can be used for detection of PUMA by Western blot at 2.5 to 5 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
PUMA Monoclonal Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMAα and PUMAβ. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Background References:
  • Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7:683-94.
  • Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001; 7:673-82.
  • Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001; 98:11318-23.
Buffer:
PUMA Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha.
NCBI Gene ID #:
27113
NCBI Official Name:
BCL2 binding component 3
NCBI Official Symbol:
BBC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9BXH1
Protein GI Number:
56748610
Purification:
PUMA Monoclonal Antibody is Protein A purified.
Research Area:
Apoptosis
Swissprot #:
Q9BXH1
User NOte:
Optimal dilutions for each application to be determined by the researcher.