AdipoGen Life Sciences

NAD Synthetase (M. tuberculosis) (rec.) (His)

Product Code:
 
AG-40T-0108
Product Group:
 
Recombinant Proteins
Antibody Clonality:
 
Enzyme
Regulatory Status:
 
RUO
Shipping:
 
Dry Ice
Storage:
 
-80°C
 

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AG-40T-0108-C05050 ug£357.00
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This product comes from: Switzerland.
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Further Information

Alternate Names/Synonyms:
NADE; Glutamine-dependent NAD(+) Synthetase; EC 6.3.5.1
Biological Activity:
~0.1 U/mg protein. (Ammonium or glutamine as nitrogen source). One unit is defined as the amount of enzyme that synthesizes 1µmol NAD per min.
Concentration:
0.5mg/ml (Lot specific)
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In 20mM TRIS-HCl, pH 7.8.
Handling Advice:
After opening, prepare aliquots and store at -80°C.Avoid freeze/thaw cycles.
Long Description:
Protein. NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag. Source: E. coli. Endotoxin content: N/A. Liquid. In 20mM TRIS-HCl, pH 7.8. Purity: >98% (SDS-PAGE). NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate
Molecular Weight:
~75.0kDa
NCBI, Uniprot Number:
P0A5L6
Package Type:
Plastic Vial
Product Description:
NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate
Purity:
>98% (SDS-PAGE)
Sequence:
NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag.
Source / Host:
E. coli
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Enzymes
UNSPSC Number:
12352204
Use & Stability:
Stable for at least 6 months after receipt when stored at -80°C.

References

Heterologous expression, purification, and enzymatic activity of Mycobacterium tuberculosis NAD(+) synthetase: M. Bellinzoni, et al.; Protein Expr. Purif. 25, 547 (2002)