Further Information
S100-B; NEF; S100Beta; S-100 protein subunit beta
1h, 10min
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample and 50µL Detection Reagent A to each well. Incubate 1 hours at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450nm immediately.
0.625-10ng/mL
48T, 96T, 96T?5, 96T?10, 96T?100
S100 Calcium Binding Protein B
Double-antibody Sandwich
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant S100 Calcium Binding Protein B (S100B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant S100 Calcium Binding Protein B (S100B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Tumor immunity;Infection immunity;Neuro science;
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
The minimum detectable dose of this kit is typically less than 0.21ng/mL
This assay has high sensitivity and excellent specificity for detection of Instant S100 Calcium Binding Protein B (S100B).
No significant cross-reactivity or interference between Instant S100 Calcium Binding Protein B (S100B) and analogues was observed.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant S100 Calcium Binding Protein B (S100B). Standards or samples and HRP-labeled detection antibody specific to Instant S100 Calcium Binding Protein B (S100B) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant S100 Calcium Binding Protein B (S100B), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Instant S100 Calcium Binding Protein B (S100B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
P04271
