Anti-CD8 (Mouse) mAb-Alexa Fluor 647

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two α chains, or a heterodimer composed of one α and one β chain. Both α and β chains share significant homology to immunoglobulin variable light chains. CD8 α chains bind to class-I MHC molecules α3 domains. CD8 identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.This monoclonal antibody KT15 reacts with a non-polymorphic epitope on the mouse CD8 α chain (mouse Ly2.1 and Ly2.2 cells).

1 mg/mL

50 ug IgG in 50 uL volume of PBS containing 1% BSA and 0.1% ProClin 150.

Mouse: 12525

This antibody reacts with mouse CD8 α antigen on Flow cytometry.

1 year

This antibody was purified from mouse ascites fluid using protein G agarose. This hybridoma (clone KT15) was established by fusion of mouse myeloma cell NSO with SD rat splenocyte immunized with the T cell clone C6, H-2Kk restricted H-Y antigen specific CTL.

CD8