NSJ Bioreagents

H3K27cr Antibody / Crotonylated-Histone H3 Lysine 27

Product Code:
 
NSJ-R20417
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RM401
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Dot Blot
  • Western Blot (WB)
Storage:
 
Store the recombinant H3K27cr antibody at -20°C.
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Western blot testing of acid extracts of HeLa cells with or without sodium crotonate treatment using recombinant H3K27cr at 0.5 ug/ml.~
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A peptide dot blot showing recombinant H3K27cr antibody reacts specifically to Histone H3 crotonylate
❮❯

Western blot testing of acid extracts of HeLa cells with or without sodium crotonate treatment using recombinant H3K27cr at 0.5 ug/ml.~
A peptide dot blot showing recombinant H3K27cr antibody reacts specifically to Histone H3 crotonylate

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CodeSizePrice
NSJ-R20417-100UG100 ug£581.00
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Further Information

Application Details:
Western blot: 0.5ug/ml-2ug/ml,Dot blot: 0.5ug/ml-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H3K27cr antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to Histone H3 crotonylated at Lysine 27 (K27cr), and does not cross-react with acetylated or butyrylated Lysine 27. No cross reactivity with other crotonylated Lysines in histone H3.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A crotonyl-peptide corresponding to Crotonyl-Histone H3 (Lys27) was used as the immunogen for the recombinant H3K27cr antibody.
Limitation:
This recombinant H3K27cr antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P84243

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