NSJ Bioreagents

H3K4me3 Antibody / Trimethyl-Histone H3 (Lys4)

Product Code:
 
NSJ-R20363
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RM340
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Co-Immunoprecipitation (Co-IP)
  • Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Store the recombinant H3K4me3 antibody at -20°C.
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SNAP-ChIP (TM) / qPCR using anti-H3K4me3 antibody. Antibody (3 ug at 17 ug/ml) was tested in native ChIP with 3 ug HEK-293 chromatin (~1x10e6 cells). Specificity (left Y-axis; all bars mean ± SEM from six independent ChIP experiments; note less than 15% cross-reactivity outside H3K4me3) was determined by qu
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SNAP-ChIP (TM) / qPCR using anti-H3K4me3 antibody. Antibody (3 ug at 17 ug/ml) was tested in native ChIP with 3 ug HEK-293 chromatin (~1x10e6 cells). Specificity (left Y-axis; all bars mean ± SEM from six independent ChIP experiments; note less than 15% cross-reactivity outside H3K4me3) was determined by qu

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NSJ-R20363-100UG100 ug£581.00
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Further Information

Application Details:
Chromatin IP: 1ug/ml-5ug/ml,ELISA: 1ug/ml-10ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant H3K4me3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This antibody reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me3), or other methylation in histone H3.
Format:
Purified
Formulation:
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Immunogen:
A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4) was used as the immunogen for the recombinant H3K4me3 antibody.
Limitation:
This recombinant H3K4me3 antibody is available for research use only.
Purity:
Protein A purified from animal origin-free supernatant
Uniprot #:
P84243

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