NSJ Bioreagents

CD99 Antibody / MIC2

Product Code:
 
NSJ-V5352
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MIC2/7867
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the CD99 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 2
Western blot testing of human MCF-7 cell lysate with CD99 antibody (clone MIC2/7867). Predicted molecular weight: 16-32 kDa depending on the level of glycosylation.~
2 / 2
IHC staining of FFPE human pancreas tissue with CD99 antibody (clone MIC2

Western blot testing of human MCF-7 cell lysate with CD99 antibody (clone MIC2/7867). Predicted molecular weight: 16-32 kDa depending on the level of glycosylation.~
IHC staining of FFPE human pancreas tissue with CD99 antibody (clone MIC2

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CodeSizePrice
NSJ-V5352-20UG20 ug£264.00
Quantity:
NSJ-V5352-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Description:
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant partial protein sequence (within amino acids 1-185) from the human protein was used as the immunogen for the CD99 antibody.
Limitation:
This CD99 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein A affinity
Uniprot #:
P14209

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