NSJ Bioreagents

CD74 Antibody

Product Code:
 
NSJ-V5110SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CLIP/8680R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the CD74 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Western blot testing of human Raji cell lysate with CD74 antibody (clone CLIP/8680R). Expected molecular weight: 33-43 kDa.~
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IHC staining of FFPE human tonsil tissue with CD74 antibody (clone CLIP/8680R). Inset: PBS used in place of primar
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Western blot testing of human Raji cell lysate with CD74 antibody (clone CLIP/8680R). Expected molecular weight: 33-43 kDa.~
IHC staining of FFPE human tonsil tissue with CD74 antibody (clone CLIP/8680R). Inset: PBS used in place of primar

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NSJ-V5110SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Description:
It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant fragment of human CD74 protein was used as the immunogen for the CD74 antibody.
Limitation:
This CD74 antibody is available for research use only.
Localization:
Cell surface, Paranuclear globular
Purity:
Protein A/G affinity
Uniprot #:
P04233

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