NSJ Bioreagents

Podoplanin Antibody / gp36

Product Code:
 
NSJ-V4700
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PDPN/7808R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the Gp36 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 1
IHC staining of FFPE human tonsil tissue with Gp36 antibody (clone PDPN/7808R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with Gp36 antibody (clone PDPN/7808R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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CodeSizePrice
NSJ-V4700-20UG20 ug£264.00
Quantity:
NSJ-V4700-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
ELISA (Order BSA-free format for coating),Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Description:
It recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN), also called Glycoprotein 36 (Gp36). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endotheliumas well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant partial protein sequence (within amino acids 1-100) from the human protein was used as the immunogen for the Gp36 antibody.
Limitation:
This Gp36 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein A/G affinity
Uniprot #:
Q86YL7

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