NSJ Bioreagents

RNF8 Antibody

Product Code:
 
NSJ-RQ7198
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RNF8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Western blot testing of 1) human HepG2, 2) human U-2 OS, 3) human 293T and 4) mouse thymus tissue lysate with RNF8 antibody. Predicted molecular weight ~56 kDa but may be observed at larger sizes due to ubiquitination.~
2 / 2
Flow cytometry testing of hum
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Western blot testing of 1) human HepG2, 2) human U-2 OS, 3) human 293T and 4) mouse thymus tissue lysate with RNF8 antibody. Predicted molecular weight ~56 kDa but may be observed at larger sizes due to ubiquitination.~
Flow cytometry testing of hum

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CodeSizePrice
NSJ-RQ7198-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the RNF8 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
E3 ubiquitin-protein ligase RNF8 is an enzyme that in humans is encoded by the RNF8 gene. The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q243-F485) was used as the immunogen for the RNF8 antibody.
Limitation:
This RNF8 antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
O76064

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