NSJ Bioreagents

Rad21 Antibody

Product Code:
 
NSJ-RQ7197
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RAD21 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human SiHa cells with RAD21 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) monkey COS-7, 2) human HeLa, 3) human MOLT-4, 4) human Jurkat, 5) human HEL, 6) human Daudi, 7) human A431, 8) human T-47D, 9) rat brain, 10) rat pancreas, 11) mouse brain and 12) mouse pancreas tissue lysate with RAD21 antibody. Expected molecular weight: ~120 kDa (full length), 64-70 kDa (cleavage product).
3 / 3
Flow cytometry testing of human HL60 cells with RAD21 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAD21 antibody.
❮❯

Immunofluorescent staining of FFPE human SiHa cells with RAD21 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) monkey COS-7, 2) human HeLa, 3) human MOLT-4, 4) human Jurkat, 5) human HEL, 6) human Daudi, 7) human A431, 8) human T-47D, 9) rat brain, 10) rat pancreas, 11) mouse brain and 12) mouse pancreas tissue lysate with RAD21 antibody. Expected molecular weight: ~120 kDa (full length), 64-70 kDa (cleavage product).
Flow cytometry testing of human HL60 cells with RAD21 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAD21 antibody.

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CodeSizePrice
NSJ-RQ7197-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the RAD21 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Double-strand-break repair protein rad21 homolog is a protein that in humans is encoded by the RAD21 gene. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D292-K529) was used as the immunogen for the RAD21 antibody.
Limitation:
This RAD21 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
O60216

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