NSJ Bioreagents

Integrin beta 3 Antibody / ITGB3 / CD61

Product Code:
 
NSJ-RQ7183
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the CD61 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
IHC staining of FFPE human lung cancer tissue with CD61 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human liver cancer tissue with CD61 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE rat lung tissue with CD61 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) human U-87 MG, 2) human HEL and 3) rat spleen lysate with CD61 antibody. Expected molecular weight: 87-110 kDa depending on glycosylation level.
5 / 5
Flow cytometry testing of human HEL cells with CD61 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD61 antibody.
❮❯

IHC staining of FFPE human lung cancer tissue with CD61 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with CD61 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat lung tissue with CD61 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human U-87 MG, 2) human HEL and 3) rat spleen lysate with CD61 antibody. Expected molecular weight: 87-110 kDa depending on glycosylation level.
Flow cytometry testing of human HEL cells with CD61 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD61 antibody.

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NSJ-RQ7183-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CD61 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
ITGB3 (INTEGRIN, BETA-3), also called GP3A, GPIIIa, CD61, is a protein that in humans is encoded by the ITGB3 gene. It is a cluster of differentiation found on thrombocytes. The 3-prime exon is larger than 1,700 nucleotides and contains the 3-prime untranslated region. The ITGB3 complex belongs to the integrin class of cell adhesion molecule receptors that share a common heterodimeric structure with alpha and beta subunits. Additionally, the ITGB3 complex mediates platelet aggregation by acting as a receptor for fibrinogen. Although the ITGB3 is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the 'inside-out' signaling pathways.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids N29-Q523) was used as the immunogen for the CD61 antibody.
Limitation:
This CD61 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P05106

Documents