NSJ Bioreagents

Prothrombin Antibody [Clone 5B6G1] (Antigen affinity purified)

Product Code:
 
NSJ-RQ6986
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
5B6G1
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the Prothrombin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Western blot testing of 1) human HeLa, 2) human HepG2, 3) human Caco-2, 4) human HL-60, 5) rat liver, 6) rat RH35, 7) mouse liver and 8) mouse HEPA1-6 cell lysate with Prothrombin antibody. Predicted molecular weight ~70 kDa but may be observed at higher molecular weights due to glycosylation.
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Western blot testing of 1) human HeLa, 2) human HepG2, 3) human Caco-2, 4) human HL-60, 5) rat liver, 6) rat RH35, 7) mouse liver and 8) mouse HEPA1-6 cell lysate with Prothrombin antibody. Predicted molecular weight ~70 kDa but may be observed at higher molecular weights due to glycosylation.

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NSJ-RQ6986-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1 ug/ml
Application Note:
Optimal dilution of the Prothrombin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. This gene for human prothrombin (F2) was assigned to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1). Thrombin also inhibits coagulation by activating protein C.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Y97-R124) was used as the immunogen for the Prothrombin antibody.
Limitation:
This Prothrombin antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P00734

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