NSJ Bioreagents

PP11 Antibody / ENDOU / Uridylate-specific endoribonuclease (Antigen affinity purified)

Product Code:
 
NSJ-RQ6982
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Uridylate-specific endoribonuclease antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
IHC staining of FFPE human lung cancer tissue with Uridylate-specific endoribonuclease antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human placental tissue with Uridylate-specific endoribonuclease antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Immunofluorescent staining of FFPE human Caco-2 cells with Uridylate-specific endoribonuclease antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 5
Western blot testing of human placental tissue with Uridylate-specific endoribonuclease antibody. Predicted molecular weight ~47 kDa.
5 / 5
Flow cytometry testing of human U-87 MG cells with Uridylate-specific endoribonuclease antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Uridylate-specific endoribonuclease antibody.
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IHC staining of FFPE human lung cancer tissue with Uridylate-specific endoribonuclease antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with Uridylate-specific endoribonuclease antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human Caco-2 cells with Uridylate-specific endoribonuclease antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human placental tissue with Uridylate-specific endoribonuclease antibody. Predicted molecular weight ~47 kDa.
Flow cytometry testing of human U-87 MG cells with Uridylate-specific endoribonuclease antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Uridylate-specific endoribonuclease antibody.

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CodeSizePrice
NSJ-RQ6982-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1 ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Uridylate-specific endoribonuclease antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Endonuclease, poly(U) specific is a protein that in humans is encoded by the ENDOU gene. This gene encodes a protein with endoribonuclease activity that binds polyuridine-enriched single-stranded RNA. This gene was initially characterized based on its high expression in placenta but was mischaracterized as a serine protease. In mouse, this gene promotes tolerance to self-antigens by regulating B cell activation-induced cell death (AICD). The protein may be useful as a tumor marker. Multiple alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K20-T386) was used as the immunogen for the Uridylate-specific endoribonuclease antibody.
Limitation:
This Uridylate-specific endoribonuclease antibody is available for research use only.
Purity:
Antigen affinity purified
Uniprot #:
P21128

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