ELK Biotechnology

Recombinant 2019-nCoV Papain-Like Protease

Product Code:
 
ELK-EPT114
Product Group:
 
Recombinant Proteins
Supplier:
 
ELK Biotechnology
Host Type:
 
Virus
Regulatory Status:
 
RUO
Shipping:
 
The product is shipped on dry ice/polar packs.
Storage:
 
Store at -70°C, stable for 6 months after receipt. Store at -70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
 

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CodeSizePrice
ELK-EPT114-50ug50ug£774.00
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This product comes from: China.
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Further Information

Accession:
QHD43415.1
Alternative Names:
Papain-like Protease; PLpro; PL-PRO; pp1a; Replicase polyprotein 1a
Apparent Molecular Weight:
34 KDa, reducing conditions
Background:
Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.
Biological Activity:
Measured by its ability to convert the fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC) to RLRGG and 7-amino-4-methylcoumarin (AMC). The Specific Activity is 241.7 pmol/min/ug.
Molecular Weight:
35.8 KDa
Purity:
Greater than 95% as determined by reducing SDS-PAGE.