ELK Biotechnology

Mouse Smad2/3 (Mothers Against Decapentaplegic Homolog 2/3) ELISA Kit

Product Code:
 
ELK9883
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Host Type:
 
Mouse
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9883-48T48T£298.00
Quantity:
ELK9883-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
Smad 2/3
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.16-10 ng/mL
Sample Type:
serum, plasma, tissue homogenates
Sensitivity:
0.058 ng/mL
Standard:
10 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Smad2/3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Smad2/3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Smad2/3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse Smad2/3 in the samples is then determined by comparing the OD of the samples to the standard curve.