ELK Biotechnology

Mouse TNFSF12 (Tumor Necrosis Factor Ligand Superfamily, Member 12) ELISA Kit

Product Code:
 
ELK9859
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Host Type:
 
Mouse
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9859-48T48T£298.00
Quantity:
ELK9859-96T96T£390.00
Quantity:
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Further Information

Alternative Names:
APO3L; DR3LG; TWEAK; APO3 ligand; TNF-related weak inducer of apoptosis
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
15.63-1000 pg/mL
Sample Type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
6.9 pg/mL
Standard:
1000 pg/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFSF12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TNFSF12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TNFSF12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse TNFSF12 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
O54907