NSJ Bioreagents

GRP94 Antibody / HSP90B1

Product Code:
 
NSJ-V2908SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rat
Antibody Isotype:
 
Rat IgG2a kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
9G10.F8.2
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the GRP94 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained with GRP94 antibody (clone 9G10.F8.2).~
2 / 2
Western blot testing of A) human HeLa and B) mouse NIH3T3 cell lysate with GRP94 antibody (clone 9G10.F8.2). Expecte
❮❯

IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained with GRP94 antibody (clone 9G10.F8.2).~
Western blot testing of A) human HeLa and B) mouse NIH3T3 cell lysate with GRP94 antibody (clone 9G10.F8.2). Expecte

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NSJ-V2908SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Optimal dilution of the GRP94 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the GRP94 antibody.
Limitation:
This GRP94 antibody is available for research use only.
Localization:
Cytoplasmic and nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
P14625

Documents