NSJ Bioreagents

Blood Group Lewis y Antibody

Product Code:
 
NSJ-V3309SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
A70-A/A9
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Lewis y antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC testing of FFPE human colon carcinoma with Lewis y antibody (clone A70-A/A9). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.~
2 / 2
SDS-PAGE analysis of purified, BSA-free Lewis y antibody (clone A70-A/A9)
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IHC testing of FFPE human colon carcinoma with Lewis y antibody (clone A70-A/A9). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.~
SDS-PAGE analysis of purified, BSA-free Lewis y antibody (clone A70-A/A9)

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NSJ-V3309SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Lewis y antibody should be determined by the researcher.
Description:
This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-). Lewis y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Live Ls174T cells (human colon carcinoma) were used as the immunogen for the Lewis y antibody.
Limitation:
This Lewis y antibody is available for research use only.
Localization:
Cell membrane, cytoplasmic and extracellular
Purity:
Protein G affinity chromatography

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