NSJ Bioreagents

AKT1 Antibody

Product Code:
 
NSJ-V8442SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
AKT1/3898R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Store the recombinant AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Western blot testing of human MCF7 cell lysate with recombinant AKT1 antibody. Predicted molecular weight ~56 kDa.~
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SDS-PAGE analysis of purified, BSA-free recombinant AKT1 antibody (clone AKT1/3898R) as confirmation of integrity and pur
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Western blot testing of human MCF7 cell lysate with recombinant AKT1 antibody. Predicted molecular weight ~56 kDa.~
SDS-PAGE analysis of purified, BSA-free recombinant AKT1 antibody (clone AKT1/3898R) as confirmation of integrity and pur

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NSJ-V8442SAF-100UG100 ug£534.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 1-2ug/ml,ELISA: order Ab without BSA for coating
Application Note:
Optimal dilution of the recombinant AKT1 antibody should be determined by the researcher.
Description:
Recognizes a protein of 62kDa, which is identified as AKT1. The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 85-189 from the human protein was used as the immunogen for the recombinant AKT1 antibody.
Limitation:
This recombinant AKT1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic, cell surface
Purity:
Protein A affinity chromatography
Uniprot #:
P31749

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