NSJ Bioreagents

Bromodeoxyuridine / BrdU Antibody

Product Code:
 
NSJ-V2322SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
BRD494
Regulatory Status:
 
RUO
Target Species:
 
Species Independent
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC staining of mouse intestine tissue (10X) with BrdU antibody (BRD494).
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IHC staining of mouse intestine tissue (10X) with BrdU antibody (BRD494).

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NSJ-V2322SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance.

1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this antibody.
Limitation:
This BrdU / Bromodeoxyuridine antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography

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