NSJ Bioreagents

CD86 Antibody

Product Code:
 
NSJ-V8523SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
C86/3713
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the CD86 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
IHC staining of FFPE human tonsil with CD86 antibody (clone C86/3713). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of human Ramos cell lysate with CD86 antibody (clone C86/3713). Expected molecular weight: 38-70 kDa depending on glycosylation level.
3 / 3
SDS-PAGE analysis of purified, BSA-free CD86 antibody (clone C86/3713) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human tonsil with CD86 antibody (clone C86/3713). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human Ramos cell lysate with CD86 antibody (clone C86/3713). Expected molecular weight: 38-70 kDa depending on glycosylation level.
SDS-PAGE analysis of purified, BSA-free CD86 antibody (clone C86/3713) as confirmation of integrity and purity.

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NSJ-V8523SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the CD86 antibody should be determined by the researcher.
Description:
Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkins disease.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 66-195 from the human protein was used as the immunogen for the CD86 antibody.
Limitation:
This CD86 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Protein G affinity chromatography
Uniprot #:
P4208

Documents